Bovine Serum Albumin Powder CAS 9048-46-8 Biochemical Reagent BSA Lyophilized Powder
Bovine Serum Albumin Powder CAS 9048-46-8 Biochemical Reagent BSA Lyophilized Powder
Bovine Serum Albumin (BSA) is a widely used blocking reagent for use in immunohistochemistry (IHC), immunocytochemistry (ICC), ELISAs and Western blotting. Used to block non-specific binding of antibodies. Bovine Serum Albumin can also be used as a protein concentration standard in Bradford assay for protein quantification. BSA is also used in cell culture.
General description
Albumin is abundantly present in human plasma. Increased levels of albumin result in dehydration. Albumin turnover is usually found in children with iron deficiency anemia. It is a predictive indicator for liver disease.
Application
Bovine Serum Albumin has been used:
to block non-specific binding of the antibodies
as a component of activating medium for inducing sperm motility
to determine the protein concentration of the bacteriocin by the bicinchoninic (BCA) method
Heat shock fractionated
Protease-free
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Features and Benefits
Preparation Note
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
