IPTG Isopropyl Β-D-Thiogalactoside CAS 367-93-1 Dioxane Free 99%
CAS No.:Formula:Appearance:Certificate:Name:Samples:Package:High Light:
| 367-93-1 | C9H18O5S | |
| White Powder | ISO9001 | |
| Isopropyl β-D-thiogalactoside | Available | |
| 100g,1kg,5kg,25kg | ||
IPTG Isopropyl Β-D-Thiogalactoside,Isopropyl Β-D-Thiogalactoside,CAS 367-93-1 | ||
IPTG CAS367-93-1 Dioxane free 99% Glycoscience Isopropyl beta-D-1-thiogalactopyranoside
| IPTG (Isopropyl β-D-thiogalactoside) Basic information |
| Product Name: | IPTG (Isopropyl β-D-thiogalactoside) |
| Synonyms: | POLYETYLENE GLYCOL 6000 MOL. BIOLOGY GRA;SULPHURIC AC ID 0,01 MOL/L 0,02 N;YPD BROTH E;Isopropyl 2,3-O-isopropylidene-4-O-benzoyl-β-L-thiorhamnopyranoside;IPTG (Isopropyl-β-D-thiogalatopyranoside);1-ISOPROPYL-BETA-D-THIOGALACTOPYRANOSIDE;isopropyl-thiogalactopyranoside;1-methylethyl1-thio-.beta.-D-Galactopyranoside |
| CAS: | 367-93-1 |
| MF: | C9H18O5S |
| MW: | 238.3 |
| : | 206-703-0 |
| Mol File: | 367-93-1.mol |
| IPTG (Isopropyl β-D-thiogalactoside) Chemical Properties |
| Melting point | 105 °C |
| alpha | -31 º (c=1, water) |
| Boiling point | 350.9°C (rough estimate) |
| density | 1.3329 (rough estimate) |
| refractive index | 1.5060 (estimate) |
| storage temp. | 2-8°C |
| solubility | Soluble in Water or Methanol. |
| pka | 13.00±0.70(Predicted) |
| form | Crystalline Powder |
| color | White |
| Water Solubility | soluble |
| Merck | 14,5082 |
| BRN | 4631 |
| Stability: | Stable. Incompatible with strong oxidizing agents. |
| InChIKey | BPHPUYQFMNQIOC-NXRLNHOXSA-N |
| CAS DataBase Reference | 367-93-1(CAS DataBase Reference) |
| EPA Substance Registry System | Isopropyl .beta.- thiogalactoside (367-93-1) |
Application
IPTG is commonly used in cloning procedures that require induction of β-galactosidase activity. It is used in conjunction with X-Gal or Bluo-Gal in blue-white selection of recombinant bacterial colonies that induce expression of the lac operon in Escherichia coli. IPTG functions by binding to the lacI repressor and altering its conformation, which prevents the repression of the β-galactosidase coding gene lacZ.
Other Notes
Non-metabolizable galactose analog.
Reconstitution
A stock solution (0.1 M) is prepared by dissolving IPTG in water with subsequent sterile filtration of the solution. The final concentration of IPTG in indicator plates should be 0.2 mM.
